Agent 47
A genetically-engineered assassin created from the recombinant DNA of five of the world's most dangerous criminals. The fact that the DNA came from multiple ethnicities allows 47 to blend in to a certain degree in most places in the world, or at least not look immediately suspicious and out-of-place. His name comes from the last two digits of a bar code on the back of his head – 640509-040147. He is a tall, bald, blue-eyed, no-nonsense individual and usually wears a suit with black leather gloves and a red tie and is known to disguise himself. Engineered from conception to be the perfect killer, 47's strength, speed, and intellect are above the human norm. According to Jacob Andersen, lead designer of Hitman 2: Silent Assassin, Agent 47 went from being "a mean old hairy guy" to having "hi-tech glasses" before getting to his current design. More inspiration came from "comic books, Hong Kong movies," and other similar media. According to Game Director Rasmus Højengaard (Hoejengaard), the idea of a clone whose future is decided by the people that created him, intrigued the Hitman team. Hojengaard felt the idea of creating the "ultimate assassin" by cloning "evolved with the character before the first Hitman game was done". 47 is modeled after David Bateson, the veteran actor who has voiced 47 in every Hitman game.
Diana Burnwood
47's handler at the Agency. Diana briefs 47 on his assignments, oversees his progress, and serves as his guide over the radio. For the vast majority of the series, 47 never sees Diana, recognizing her only by voice, however, they do come face-to-face at the conclusion of Hitman: Blood Money and Hitman: Contracts. Diana has an upper-class English accent and a business-like demeanor. Although Diana usually handles 47's affairs from afar, she turns out to be a major character in Hitman: Blood Money; serving as a double agent, faking 47's death, saving the Agency from liquidation, and dodging death herself a few times. In the end, her efforts pay off when the Agency is brought back online, although it is left unmentioned if Diana has been promoted to a higher position. Diana is voiced by Vivienne McKee. In the Hitman film, Diana's only form of contact with 47 is through a synthesized voice via laptop. However, she does directly call 47 by phone in order to warn him that the Organization has targeted him.
Agent Smith
A fairly inept American CIA agent with ties to the Agency. He has a tendency to be captured and tortured by the people he has been assigned to spy on. As a result, the Agency has sent 47 to rescue him on a number of occasions. His torture experiences have caused him to take up drinking on the job, making him even more ineffective. Like 47, Agent Smith dons a variety of disguises, but most often he's seen beaten and stripped down to his American Flag boxer shorts. He seems to regard 47 as a friend, no doubt because 47 has rescued him so many times. The feeling is not mutual. 47 initially treats his relationship with Agent Smith as detached and professional, but as he is forced to rescue Smith again and again he increasingly sees Smith as an ineffective hindrance. In Hitman: Blood Money, 47 puts a gun to Smith's head and threatens to kill him. Smith's appearance changes somewhat in Hitman: Blood Money, having lost most of his hair through long term alcoholism and depression. In the Hitman film, Smith is instead portrayed as a very competent and dutiful CIA agent. In return for assassinating a high priority CIA target (Udre Belicoff, the brother of 47's original target), Smith helps 47 elude arrest by Interpol.
Mei-Ling
A young woman from the Chinese mainland, abducted and recruited into the brothel of Hong Kong crime lord Lee Hong. 47 rescues her in exchange for information about her employer in preparation for assassinating him. After escaping from Lee Hong, Mei-Ling is later revealed to be prostituting herself to Hayamoto, another Asian crime lord. 47 ends up rescuing her again (much to his consternation). Lei-Ling is apparently the first woman who has ever kissed 47; his reaction is different in the original game from the remake. In the original Hitman, 47 reacts with significant revulsion. In the remake Hitman: Contracts, his reaction is one of detached bemusement. She was known as Lei Ling in the original Hitman.
Dr. Otto Wolfgang Ort-Meyer
47's creator and the ultimate villain of Hitman: Codename 47. A brilliant but dangerous individual. Ostracized by the scientific community for his radical theories on genetic manipulation, Ort-Meyer ran a sophisticated lab hidden beneath a Romanian asylum. There he labored on DNA splicing in an effort to create a flawless human being. He took a great part in raising and indoctrinating 47 at the asylum, where 47 was kept a prisoner until he matured. Ort-Meyer eventually engineered 47's "escape" from the asylum in order to test his performance in the real world. The consummate megalomaniac, Ort-Meyer contracted with the Agency to have 47 kill the other 4 men behind the Hitman Project (47's "fathers"), so that Ort-Meyer could enjoy the fruits of his labor by himself. Ort-Meyer then attempted to lure 47 to his death, having perfected "Mr. 48", an improved, mindlessly loyal series of clones. Ort-Meyer underestimated how far 47 had advanced, and in the final showdown, 47 slaughtered the 48s and then killed Ort-Meyer himself by snapping his neck.